Electrophoresis: menene menene, menene don kuma yadda ake aikata shi

Wadatacce

• Menene don
• Yadda ake yinta
• Ire-iren electrophoresis
• 1. Hemoglobin electrophoresis
• 2. Amintaccen electrophoresis

Electrophoresis fasaha ce ta dakin gwaje-gwaje wacce aka yi ta da manufar raba kwayoyin gwargwadon girmansu da cajin lantarki ta yadda za'a iya gano cututtukan, a tabbatar da bayanin sunadarai ko kuma za'a iya gano kananan halittu.

Electrophoresis hanya ce mai sauƙi da arha, ana amfani da ita cikin ayyukan dakunan gwaje-gwaje da kuma ayyukan bincike. Dangane da manufar electrophoresis, yana iya zama dole don yin wasu gwaje-gwaje da gwaje-gwaje don isa ga ganewar asali, misali.

Menene don

Za'a iya yin amfani da wutar lantarki don dalilai da yawa, duka a cikin ayyukan bincike da kuma ganewar asali, tunda fasaha ce mai sauƙi da arha.Don haka, ana iya yin zaɓin lantarki zuwa:

• Gano ƙwayoyin cuta, fungi, ƙwayoyin cuta da ƙwayoyin cuta, tare da wannan aikace-aikacen ya zama gama gari a cikin ayyukan bincike;
• Gwajin uba;
• Duba maganganun sunadarai;
• Gano maye gurbi, kasancewa mai amfani a cikin cutar sankarar bargo, misali;
• Binciki nau'ikan yaduwar haemoglobin, kasancewar kuna da amfani wajen gano cutar rashin lafiya ta sikila;
• Kimanta yawan sunadaran da ke cikin jini.

Dangane da manufar electrophoresis, yana iya zama dole don gudanar da wasu ƙarin gwaje-gwaje don likita don kammala binciken.

Yadda ake yinta

Don yin electrophoresis ya zama dole gel, wanda zai iya zama na polyacrylamide ko agarose dangane da maƙasudin, maƙallan electrophoresis da vat, alamar alamomin kwayoyin da fenti mai haske, ban da UV ko kayan wuta mai haske, wanda aka fi sani da transilluminator .

Bayan shirya gel, dole ne a sanya takamaiman abu don yin rijiyoyin a cikin gel, wanda aka fi sani da tsefe, kuma bari gel ɗin ya kafa. Lokacin da gel din ya shirya, yi amfani da abubuwan kawai zuwa rijiyoyin. Saboda wannan, dole ne a sanya alamar nauyi ta kwayoyin a ɗayan rijiyoyin, sarrafa mai kyau, wanda shine abin da aka san abin da yake, sarrafawa mara kyau, wanda ke ba da tabbacin ingancin aikin, da samfuran da za a bincika. Duk samfuran dole ne a haɗe su da fenti mai kyalli, saboda ta wannan hanyar yana yiwuwa a hango makada a kan transilluminator.

Dole ne a sanya gel ɗin tare da samfuran a cikin wutar lantarki, wanda ya ƙunshi takamaiman abin da za a yi amfani da shi, sa'annan a kunna na'urar ta yadda akwai wutan lantarki kuma, sakamakon haka, yuwuwar bambance-bambancen, wanda ke da mahimmanci don rabuwa da ƙwayoyin to kayansu da girmansu. Lokacin gudu na lantarki yana canzawa gwargwadon manufar aikin, kuma zai iya wucewa zuwa awa 1.

Bayan lokacin da aka ƙayyade, yana yiwuwa a duba sakamakon zaɓin zaɓin lantarki ta hanyar transilluminator. Lokacin da aka sanya gel a ƙarƙashin UV ko hasken LED, zai yuwu a ga tsarin haɗi: ya fi girma kwayar, ƙananan ƙaurarsa, kusantar rijiyar, yayin da kwayar ta ƙara haske, mafi girman ƙarfin ƙaura.

Don amsawa don inganta, ya zama dole a nuna maɗaura na kyakkyawan iko kuma cewa a cikin mummunan iko babu abin da ake gani, saboda in ba haka ba yana nuni ne cewa akwai cuta, kuma dole ne a maimaita dukkan aikin.

Ire-iren electrophoresis

Ana iya yin electrophoresis don dalilai daban-daban kuma, bisa ga ma'anarta, ana iya amfani da nau'ikan gel da yawa, mafi mahimmanci shine polyacrylamide da agarose.

Electrophoresis don gano ƙananan ƙwayoyin cuta ya fi yawa a cikin dakunan binciken bincike, duk da haka, don dalilan bincike, ana iya amfani da electrophoresis don gano cututtukan jini da cututtukan da ke faruwa tare da ƙaruwar adadin sunadarai, kasancewar sune manyan nau'ikan electrophoresis:

1. Hemoglobin electrophoresis

Hemoglobin electrophoresis fasaha ce ta dakin gwaje-gwaje da aka gudanar don gano nau'ikan nau'ikan haemoglobin da ke yawo a cikin jini, wanda hakan ke ba da damar gano kasancewar cututtukan da suka shafi haduwar haemoglobin. An gano nau'in haemoglobin ta hanyar electrophoresis a wani takamaiman pH, daidai tsakanin 8.0 da 9.0, tare da wani tsari na bango da ake tabbatar da shi wanda za'a iya kwatanta shi da yanayin yau da kullun, yana ba da damar sanin kasancewar haemoglobins mara kyau.

Abin da aka yi don: Hemoglobin electrophoresis ana yin sa ne don bincike da kuma gano cututtukan da suka danganci haɗakar haemoglobin, kamar cutar sikila da cutar haemoglobin C, ban da kasancewarsu masu amfani wajen bambance thalassaemia. Koyi yadda ake fassara electromhoresis na haemoglobin.

2. Amintaccen electrophoresis

Protein electrophoresis gwaji ne da likita ya nema don tantance adadin sunadaran da ke yawo a cikin jini kuma, don haka, don gano cututtuka. Ana yin wannan gwajin ne daga samfurin jini, wanda aka dasa shi don samun jini, wanda wani sashi na jini, wanda ya hada, da sauran abubuwa, na sunadarai.

Bayan electrophoresis, ana iya ganin wani tsari na makada kuma, daga baya, hoto ne wanda ake nuna yawan kowane bangare na sunadarai, kasancewar shine asalin ganowar.

Abin da aka yi don: Amintaccen electrophoresis yana bawa likita damar bincikar abin da ya faru na yawan myeloma, rashin ruwa, cirrhosis, kumburi, cutar hanta, pancreatitis, lupus da hauhawar jini bisa ga tsarin band da kuma jadawalin da aka gabatar a rahoton binciken.

Fahimci yadda ake aikatawa da yadda za'a fahimci sakamakon furotin electrophoresis.